Last Order is the 20001st Sister, a clone of Misaka Mikoto, that was produced as part of the Sisters Project for the Level 6 Shift Project. She becomes an integral part of the Toaru Majutsu no Index storyline after meeting with Accelerator who later dedicates himself to protecting her. Last Order is depicted as a 10-year old version of Mikoto with one unusual strand of hair (known as an ahoge) that acts up in humorous moments in the manga. In her debut appearance, she's seen wearing a ragged piece of blanket as makeshift clothes. She is later given proper attire after being released from the hospital, wearing a blue spotted dress with a white coat and sandals. Unlike the other clones, her eyes are the same color as the original. Like Mikoto, Last Order has a complex in regards to her chest size and is easily upset about it. Unlike her clone sisters, she is capable of actually showing emotions and has a close relationship with Misaka 10032, Accelerator and her adult guardians, Yomikawa Aiho and Yoshikawa Kikyou. Like her older clone sisters, she speaks in third person, except she says "Misaka" twice. She has all of the memories of the Misaka clones and has observed Accelerator through this method during the experiment, posing a theory that Accelerator did not actually want to take part in the Level 6 Shift Project and therefore doesn't blame him for his actions. She also has an apparent admiration of Touma due to him stopping the experiments and also protecting her during the events of volume 13. Her appearance matches her childish nature, being playful and curious, such as taking Misaka 10032's goggles to play with as well as playing tag with her. She is also very caring as seen in New Testament, when she puts her life on the line to save Fräulein Kreutune, despite the latter wanting to devour her brain. Though only being a child, she is shown to be very perceptive, as demonstrated when she theorizes that Accelerator did not want to take part in the Level 6 Shift Project.
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